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Identification of ‘ C andidatus phytoplasma ziziphi’ associated with persimmon ( D iospyros kaki T hunb.) fasciation in C hina
Author(s) -
Wang J.,
Gao R.,
Yu X. M.,
An M.,
Qin Z. H.,
Liu J.,
Ai C. X.
Publication year - 2015
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12194
Subject(s) - diospyros kaki , biology , restriction fragment length polymorphism , phytoplasma , 16s ribosomal rna , phylogenetic tree , gene , ribosomal rna , polymerase chain reaction , microbiology and biotechnology , genetics , botany
Summary Persimmon ( Diospyros kaki Thunb.) trees, with fasciation symptoms ( PF ), were observed in an orchard located in suburban Tai'an, Shandong Province, China. A specific fragment of the phytoplasma 16S rRNA gene, approximately 1.2 kb in length, was amplified from two symptomatic plants via nested polymerase chain reaction, while no fragment was obtained from healthy controls. The two samples ( PF 1 and PF 2) resulted with 99.5% nucleotide sequence identity. Phylogenetic and restriction fragment length polymorphism ( RFLP ) analysis of 16S rRNA gene sequences revealed that PF 1 was a member of ribosomal subgroup B of the elm yellows group (16SrV), and PF 2 may represent a novel subgroup within the 16SrV group, designed as 16SrV‐I. This is the first report of phytoplasmas of the 16SrV group associated with persimmon fasciation disease.