Premium
A molecular diagnostic assay for the detection and identification of wood decay fungi of conifers
Author(s) -
Gonthier P.,
Guglielmo F.,
Sillo F.,
Giordano L.,
Garbelotto M.
Publication year - 2015
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12132
Subject(s) - biology , multiplex , heterobasidion annosum , sensu , botany , internal transcribed spacer , polymerase chain reaction , multiplex polymerase chain reaction , microbiology and biotechnology , picea abies , gene , genetics , ribosomal rna , genus
Summary Ten taxon‐specific primers were designed to amplify the Internal Transcribed Spacer of the r RNA operon of several important decay fungi of coniferous wood, including A rmillaria spp., E chinodontium spp., F omitopsis pinicola , F uscoporia torulosa , H eterobasidion annosum sensu lato ( s.l .), O nnia spp., P haeolus schweinitzii , P hellinus weirii s.l ., P holiota spp. and P orodaedalea spp. Primers designed in this study and in a previous one for the identification of L aetiporus sulphureus and S tereum spp. were combined in two multiplex PCR s, which were tested for efficiency and specificity, and detected at least 1 pg of fungal target DNA . Target DNA at concentrations of 10 −1 pg or lower can be detected with this assay using SYBR ® G reen R eal‐ T ime PCR . Validation assays performed on 129 naturally infected wood samples or fruiting bodies confirmed the reliability of the multiplex PCR ‐based diagnostic method. This method represents a simple and rapid diagnostic tool for the detection of a number of destructive wood decay fungi of conifer wood.