Premium
Occurrence of ‘ Candidatus Phytoplasma ziziphi’ in apple trees in C hina
Author(s) -
Li Z.N.,
Bai Y.B.,
Liu P.,
Zhang L.,
Wu Y.F.
Publication year - 2014
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12126
Subject(s) - phytoplasma , biology , restriction fragment length polymorphism , sieve tube element , polymerase chain reaction , nested polymerase chain reaction , botany , horticulture , gene , genetics , phloem
Summary Young apple trees exhibiting symptoms of little leaf, margin involute and yellows were observed in an open‐air nursery in Yangling, Shaanxi, China. Transmission electron microscopy showed typical phytoplasma bodies in the sieve tube elements of symptomatic leaf samples. In two nested polymerase chain reaction ( PCR ) assays, products of expected size of 1.1 and 1.2 kb were separately generated from the total DNA s of symptomatic samples. A restriction fragment length polymorphism ( RFLP ) analysis of the purified 1.2 kb PCR products indicated that the disease associated with apple trees was ‘ Ca . Phytoplasma ziziphi’. To our knowledge, this is the first report of ‘ Ca . Phytoplasma ziziphi’ infecting apple trees in China.