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Species distribution, host affinity and genetic variability of H eterobasidion annosum sensu lato in the C zech R epublic
Author(s) -
Sedlák P.,
Tomšovský M.
Publication year - 2014
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12102
Subject(s) - biology , heterobasidion annosum , botany , picea abies , sensu , genus
Summary The distribution and host affinity of Heterobasidon annosum (Fr.) Bref. sensu lato (Basidiomycota, Bondarzewiaceae ) in the Czech Republic was investigated. Sampling was undertaken in natural forests, in stands with former presence of fir and spruce and in forests on formerly agricultural lands. The identification and phylogenetic relationship of the species complex was studied by comparing DNA sequences of three gene regions: glyceraldehyde 3‐phosphate dehydrogenase (G3P), translation elongation factor 1‐alfa (EFA) and transcription factor (TF). The species relationships were demonstrated by haplotype network and Bayesian phylogram construction for the combined data set. The distribution of each species was demonstrated. Heterobasidion annosum s.s., which had the greatest host range, was found on thirteen genera ( Pinus, Picea, Fraxinus, Betula, Corylus, Alnus, Abies, Acer, Salix, Ligustrum, Quercus, Larix and Prunus ). Heterobasidion parviporum was found on spruce ( Picea abies ), fir ( Abies alba ), apple tree ( Malus sylvestris ), Vitis vinifera and Fagus sylvatica, and Heterobasidion abietinum was observed on fir ( Abies ), spruce ( Picea ), pine ( Pinus ) and maize ( Zea mays ). Sequences from different genes yielded conflicting results for six specimens, which were interpreted as belonging to interspecific hybrids. These were collected from A. alba, P. abies and Chamaecyparis lawsoniana . The occurrence of Heterobasidion annosum s.l. is strongly associated with the natural distribution of its hosts and the occurrence of H. abietinum appears to be related to the historical occurrence of A. alba in stands now reforested with P. abies . No statistically significant differences in the vertical distributions within the species complex were detected.