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Screening for resistant clones of C asuarina equisetifolia to bacterial wilt and the analysis of AFLP markers in resistant clones
Author(s) -
Sun S.,
Shu C. W.,
Chen J. L.,
Wang J.,
Zhou E. X.
Publication year - 2014
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12097
Subject(s) - biology , amplified fragment length polymorphism , inoculation , genbank , botany , genetic marker , accession number (library science) , horticulture , genetics , genetic diversity , gene , population , demography , sociology
Summary Bacteria wilt ( BW ) of C asuarina equisetifolia L . Johnson, caused by R alstonia solanacearum ( E . F . S mith) Y abuuchi, is one of the most important diseases of C . equisetifolia L . J ohnson, the main coastal protective forest tree in southeast C hina. The resistance of 33 C . equisetifolia L . J ohnson clones to BW was determined by means of hydroponic and pot inoculation methods with the suspension of R solanacearum ( E . F . S mith) Y abuuchi as inocula in the two‐step procedure. The results showed that clones FJ 11 and FJ 13 were highly resistant to BW with the lowest disease incidences of 10.8 and 15.8%, respectively, whereas clones FJ 9 and GD 15 were the most susceptible ones with the highest disease incidences of 100.0%. The analysis of AFLP markers was performed on clones FJ 11, FJ 13, FJ 9 and GD 15, and the results indicated that three DNA fragments E ‐ AAA /M‐ CCG , E‐ AAG / M ‐ CGC and E ‐ AGG / M ‐ CAA were found to be linked to the BW resistance. BLAST searches revealed that the first two fragments (GenBank accession numbers: KC 111411 and KC 135871) had no identities to any sequences, and the third one showed a high similarity to some sequences which code for heat shock protein. Taken together, the two resistant clones screened in our research were undoubtedly the valuable resistant resources of C . equisetifolia L . J ohnson, and the three AFLP markers could be used in marker‐assisted selection of C . equisetifolia L . J ohnson in the future.