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Identification and molecular characterization of 16 S r V group phytoplasmas with flowering cherry in C hina
Author(s) -
Wang L.P.,
Hong N.,
Xu W.X.,
Wang Y.F.,
Chofong G.N.,
Martini M.,
Su J.M.,
Wang G.P.
Publication year - 2014
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12092
Subject(s) - biology , restriction fragment length polymorphism , phytoplasma , phylogenetic tree , restriction enzyme , botany , operon , gene , ribosomal rna , polymerase chain reaction , genetics , escherichia coli
Summary Flowering cherry ( P runus serrulata ) trees showing symptoms of excessive axillary growth, stunting, short internodes and decline were observed in Y antai C ity, S handong province, C hina. The samples with the flowering cherry decline were designated as F c D ‐ YT phytoplasma. Sequencing results, restriction fragment length polymorphism ( RFLP ) and phylogenetic analysis of sequences from the 1248‐bp R 16 F 2n⁄ R 16 R 2 products and partial ribosomal protein operon genes revealed the F c D ‐ YT phytoplasma as a member of subgroup 16 S r V ‐ B , rp V ‐ C . Two RFLP patterns were observed in recombinant colonies from the cloned 16 S r DNA gene with endonucleases H pa II and M se I . To our knowledge, this is the first report of 16 S r V group phytoplasma declining flowering cherry trees in China.