Identification and molecular characterization of 16 S r V  group phytoplasmas with flowering cherry in  C hina | Zendy

Wang L.P. | Zendy; Hong N. | Zendy; Xu W.X. | Zendy; Wang Y.F. | Zendy; Chofong G.N. | Zendy; Martini M. | Zendy; Su J.M. | Zendy; Wang G.P. | Zendy

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Identification and molecular characterization of 16 S r V group phytoplasmas with flowering cherry in C hina

Author(s) -

Wang L.P.,

Hong N.,

Xu W.X.,

Wang Y.F.,

Chofong G.N.,

Martini M.,

Su J.M.,

Wang G.P.

Publication year - 2014

Publication title -

forest pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.535

H-Index - 49

eISSN - 1439-0329

pISSN - 1437-4781

DOI - 10.1111/efp.12092

Subject(s) - biology , restriction fragment length polymorphism , phytoplasma , phylogenetic tree , restriction enzyme , botany , operon , gene , ribosomal rna , polymerase chain reaction , genetics , escherichia coli

Summary Flowering cherry ( P runus serrulata ) trees showing symptoms of excessive axillary growth, stunting, short internodes and decline were observed in Y antai C ity, S handong province, C hina. The samples with the flowering cherry decline were designated as F c D ‐ YT phytoplasma. Sequencing results, restriction fragment length polymorphism ( RFLP ) and phylogenetic analysis of sequences from the 1248‐bp R 16 F 2n⁄ R 16 R 2 products and partial ribosomal protein operon genes revealed the F c D ‐ YT phytoplasma as a member of subgroup 16 S r V ‐ B , rp V ‐ C . Two RFLP patterns were observed in recombinant colonies from the cloned 16 S r DNA gene with endonucleases H pa II and M se I . To our knowledge, this is the first report of 16 S r V group phytoplasma declining flowering cherry trees in China.

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