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Development of a quantitative real‐time PCR assay for the detection of P hytophthora austrocedrae , an emerging pathogen in B ritain
Author(s) -
Mulholland V.,
Schlenzig A.,
MacAskill G. A.,
Green S.
Publication year - 2013
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12058
Subject(s) - biology , pathogen , microbiology and biotechnology , juniper , real time polymerase chain reaction , dna , gene , genetics , botany
Summary A T aq M an real‐time PCR assay was developed for P hytophthora austrocedrae, an emerging pathogen causing severe damage to juniper in B ritain. The primers amplified DNA of the target pathogen down to 1 pg of extracted DNA , in both the presence and absence of host DNA , but did not amplify any of the non‐target P hytophthora and fungal species tested. The assay provides a useful tool for screening juniper populations for the disease.