Development of a quantitative real‐time  PCR  assay for the detection of   P hytophthora austrocedrae , an emerging pathogen in  B ritain | Zendy

Mulholland V. | Zendy; Schlenzig A. | Zendy; MacAskill G. A. | Zendy; Green S. | Zendy

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Development of a quantitative real‐time PCR assay for the detection of P hytophthora austrocedrae , an emerging pathogen in B ritain

Author(s) -

Mulholland V.,

Schlenzig A.,

MacAskill G. A.,

Green S.

Publication year - 2013

Publication title -

forest pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.535

H-Index - 49

eISSN - 1439-0329

pISSN - 1437-4781

DOI - 10.1111/efp.12058

Subject(s) - biology , pathogen , microbiology and biotechnology , juniper , real time polymerase chain reaction , dna , gene , genetics , botany

Summary A T aq M an real‐time PCR assay was developed for P hytophthora austrocedrae, an emerging pathogen causing severe damage to juniper in B ritain. The primers amplified DNA of the target pathogen down to 1 pg of extracted DNA , in both the presence and absence of host DNA , but did not amplify any of the non‐target P hytophthora and fungal species tested. The assay provides a useful tool for screening juniper populations for the disease.

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