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Genetic characterization of B otrytis cinerea isolates collected from pine and eucalyptus nurseries in B io‐ B io Region, C hile
Author(s) -
Muñoz G.,
Campos F.
Publication year - 2013
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12057
Subject(s) - biology , eucalyptus globulus , radiata , transposable element , fungicide , pinus radiata , botany , ceratocystis , eucalyptus , microbiology and biotechnology , mutant , genetics , gene , fungus , vigna
Summary A total of 55 B otrytis cinerea isolates collected from P inus radiata and E ucalyptus globulus plants cultivated in nurseries located in the Bio‐Bio Region, Chile, as well as isolates collected from native plants such as Rubus spp and A ristotelia chilensis located near the nurseries were genetically characterized. All isolates carried the Bc‐hch 2 allele, thus belonging to genetic Group II, which is now referred to as B . cinerea . Genotyping based on the presence of transposons Boty and Flipper showed differences between isolates related to the plant host. Thus, transposa isolates (containing both transposons) were detected in P . radiata and E . globulus, while vacuma isolates (containing neither transposon) were detected in all plants except E . globulus . Notably, boty isolates (containing just the Boty transposon) were detected at high frequencies in all plant hosts. Analyses to detect mutations involved in resistance to fungicides such as benzimidazoles (BZ), dicarboximides and QoIs also showed differences in the studied isolates. Isolates collected from E . globulus were shown to carry mutations for all tree fungicides, while those collected from P . radiata presented mutations involved in resistance to BZ only. Isolates collected from native plant hosts did not carry any of the mutations analysed.