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Aseptic B ursaphelenchus xylophilus does not reduce the mortality of young pine tree
Author(s) -
Zhao H.,
Chen C.,
Liu S.,
Liu P.,
Liu Q.,
Jian H.
Publication year - 2013
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12052
Subject(s) - bursaphelenchus xylophilus , xylophilus , biology , inoculation , aseptic processing , nematode , bacteria , botany , wilt disease , horticulture , microbiology and biotechnology , veterinary medicine , ecology , food science , medicine , genetics
Summary To assess the role of bacteria in pine wilt disease ( PWD ), aseptic M form (with a mucronated tail) and R form (with a round tail) of B ursaphelenchus xylophilus and B . mucronatus were obtained and compared, in terms of reproduction and pathogenicity, with non‐aseptic nematode. In addition, bacteria isolated from non‐aseptic nematodes and pine trees inoculated with non‐aseptic nematodes were identified. The results indicated that the bacteria associated with nematodes significantly lowered the reproduction of R form of B . xylophilus and B . mucronatus . Both the non‐aseptic and aseptic R forms of B . xylophilus induced death in all infected 7‐ to 8‐year‐old pine trees, while the non‐aseptic and aseptic M forms of B . xylophilus and B . mucronatus caused almost no plant mortality. High numbers of the non‐aseptic and aseptic R forms of B . xylophilus were distributed throughout the inoculated trees, while B . mucronatus and M form of B . xylophilus nematodes were lower in number and their distribution in stems limited within the inoculation site. Bacteria isolated from non‐aseptic nematodes were not recovered from the pine trees inoculated with these same kinds of nematodes. Two species of bacteria were both isolated from non‐aseptic B . mucronatus and from R form of B . xylophilus . M icrobacterium trichotecenolyticum was common to both the control and inoculated pine trees. These results suggest that R form of B . xylophilus is the causal agent of PWD and that bacteria cannot increase the virulence of B . xylophilus and B . mucronatus .