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On the track of B ursaphelenchus pinophilus Brzeski and Baujard, 1997 ( N ematoda: A phelenchoididae) in P ortugal
Author(s) -
Vieira P.,
Mota M.
Publication year - 2013
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12050
Subject(s) - biology , internal transcribed spacer , spicule , ribosomal dna , microbiology and biotechnology , ribosomal rna , anatomy , genetics , gene , phylogenetics , sponge spicule
Summary This is the first report and characterization of B ursaphelenchus pinophilus in P ortugal. This species was isolated from a young dying P inus pinaster tree located in V alverde, in the A lentejo region. Nematodes were identified using several morphological diagnostic characters for this species (male spicule structure, number of lateral incisures, number and distribution of the male papillae, presence of female vulval flap and female tail shape) and confirmed using RFLP analysis of the internal transcribed spacer ( ITS ) regions of ribosomal DNA .