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Detection and genetic variability of E uropean mountain ash ringspot‐associated virus ( EMAR a V ) in S weden
Author(s) -
Bargen S.,
Arndt N.,
Robel J.,
Jalkanen R.,
Büttner C.
Publication year - 2013
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12046
Subject(s) - biology , virus , coding region , virology , botany , gene , genetics
Summary European mountain ash ringspot‐associated virus ( EMAR a V ) is a plant virus inducing characteristic ringspots and mottling in S orbus aucuparia L . For the first time, EMAR a V was detected in mountain ash in S weden. All four genomic segments of the virus were detectable by RT ‐ PCR after total RNA extraction from leaves showing chlorotic ringspots, mottling or necrotic lesions. The samples originated from southern and northern S weden. Sequence analyses of amplified fragments revealed low genetic variability of the virus at nucleotide as well as protein level. All investigated coding regions of EMAR a V were under strong purifying selection pressure.