Premium
Analysis of microtubule and microfilament distribution in Pinus sylvestris roots following infection by Heterobasidion species
Author(s) -
Zadworny M.,
Guzicka M.,
Łakomy P.,
Samardakiewicz S.,
Smoliński D. J.,
Mucha J.
Publication year - 2013
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12017
Subject(s) - biology , microtubule , cytoskeleton , heterobasidion annosum , inoculation , botany , microfilament , pinus <genus> , host (biology) , microbiology and biotechnology , actin , cell , genetics , horticulture , picea abies
Summary Cytoskeletal dynamics play a crucial role in pathogen recognition and cell defence during the initial interactions between an invader and plant host. The aim of the work reported here was to characterize how H eterobasidion annosum s.s., H eterobasidion parviporum , and H eterobasidion abietinum affect the microtubules and microfilaments of P inus sylvestris root cells 12‐, 24‐, 48‐, and 96‐h post‐inoculation. Inoculation of P . sylvestris with H . parviporum or H . abietinum , which have a lower specificity for P . sylvestris than H . annosum s.s, resulted in greater reorganization of host microtubules during the early stages of interaction than inoculation with the more specific H . annosum s.s. In some infected cells, spots of actin aggregates were observed. Disruption of cytoskeletal components by the application of specific cytoskeletal inhibitors facilitated the entry of the H . parviporum and H . abietinum into roots. These results suggest that the P . sylvestris cytoskeleton plays a role in the host response in the initial stages of the host–pathogen interaction.