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An objective high‐throughput screening method for thrips damage quantitation using Ilastik and ImageJ
Author(s) -
Visschers Isabella G. S.,
Dam Nicole M.,
Peters Janny L.
Publication year - 2018
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1111/eea.12682
Subject(s) - thrips , biology , thripidae , pepper , horticulture , cultivar , pest analysis , resistance (ecology) , botany , agronomy
Quantifying thrips damage is challenging. We present an automated protocol for the screening of thrips (Thysanoptera: Thripidae) damage on Capsicum (pepper) leaves. We show that standardizing the exposed leaf side is important for resistance ranking of accessions. Our results indicate that thrips resistance is dependent on the plant's developmental stage and its genetic background. Ultimately, our method provides an efficient tool for screening for resistance to insects in applied research and commercial breeding.