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Effect of Cry1F maize on the behavior of susceptible and resistant S podoptera frugiperda and O strinia nubilalis
Author(s) -
Vélez Ana María,
Alves Analiza P.,
Blankenship Erin E.,
Siegfried Blair D.
Publication year - 2016
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1111/eea.12409
Subject(s) - ostrinia , biology , spodoptera , lepidoptera genitalia , larva , pyralidae , pest analysis , fall armyworm , bacillus thuringiensis , european corn borer , noctuidae , botany , horticulture , biochemistry , genetics , gene , bacteria , recombinant dna
Understanding the behavior of pests targeted with B acillus thuringiensis B erliner (Bt) crops is important to define resistance management strategies. Particularly the study of larval movement between plants is important to determine the feasibility of refuge configurations. Exposure to Bt maize, Z ea mays L . ( P oaceae), has been suggested to increase larval movement in lepidopteran species but few studies have examined the potential for resistance to interact with behavioral responses to Bt toxins. Choice and no‐choice experiments were conducted with S podoptera frugiperda ( JE S mith) ( L epidoptera: N octuidae) and O strinia nubilalis ( H übner) ( L epidoptera: C rambidae) to determine whether Cry1F resistance influences neonate movement. Leaf discs of Cry1F maize and the corresponding isoline were used to characterize behavioral responses. In both experiments, the location (on or off of plant tissues) and mortality of susceptible and Cry1F resistant neonates was recorded for 5 days, but the analysis of larvae location was performed until 7 h. Our results indicated no strong difference between resistant and susceptible phenotypes in S . frugiperda and O . nubilalis , although a small percentage of susceptible neonates in both species abandoned maize tissue expressing Cry1F. However, significant behavioral differences were observed between species. O strinia nubilalis exhibited increased movement between leaf discs, whereas S . frugiperda selected plant tissue within the first 30 min and remained on the chosen plant regardless of the presence of Cry1F. S podoptera frugiperda reduced larval movement may have implications to refuge configuration. This study represents the first step toward understanding the effects of Cry1F resistance on L epidoptera larval behavior. Information regarding behavioral differences between species could aid in developing better and more flexible resistance management strategies.