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Transcriptomic responses to different doses of cycloxaprid involved in detoxification and stress response in the whitebacked planthopper, S ogatella furcifera
Author(s) -
Yang Yuanxue,
Zhang Yixi,
Yang Baojun,
Fang Jichao,
Liu Zewen
Publication year - 2016
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1111/eea.12406
Subject(s) - biology , carboxylesterase , cytochrome p450 , transcriptome , brown planthopper , monooxygenase , toxicology , gene , detoxification (alternative medicine) , imidacloprid , semiochemical , pesticide resistance , xenobiotic , gene expression , pest analysis , genetics , pesticide , biochemistry , botany , enzyme , medicine , alternative medicine , pathology , agronomy
The whitebacked planthopper, S ogatella furcifera ( H orváth) ( H emiptera: D elphacidae), an economically important rice pest, has developed resistance to many insecticides. Cycloxaprid, a novel cis ‐nitromethylene neonicotinoid insecticide, has good industrialization prospects because of its high efficiency against rice planthoppers, including imidacloprid‐resistant populations. This study focused on the genes involved in xenobiotic detoxification and stress reaction after exposure to doses of cycloxaprid. Ten cytochrome P450 monooxygenase genes (one CYP 3, three CYP 4, and six CYP 6), one microsomal glutathione S‐transferase ( GST ) gene, and one acetylcholinesterase ( AC hE) gene were up‐regulated in response to a high‐dose treatment ( HD , 85% mortality). Five P450 genes (one CYP 4, two CYP 6, and one CYP 15), one delta‐ GST gene, and two carboxylesterase genes were up‐regulated in response to a low‐dose treatment ( LD , 15% mortality). These findings were consistent with previous reports showing that CYP 4, CYP 6, and GST d were associated with insecticide detoxification in insects and contributed significantly to insecticide resistance. Another interesting finding was that one Hsp70 and one small Hsp ( sH sp) were up‐regulated in response to HD treatment, whereas they were downregulated in response to LD treatment. In order to check the accuracy of transcriptome data, 10 unigenes were randomly selected to compare their expression between treatments. Regulations of related genes in response to cycloxaprid doses could contribute to explaining the quick responses to insecticide stress.