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Characterization of the feeding behavior of three E rythroneura species on grapevine by histological and DC ‐electrical penetration graph techniques
Author(s) -
Saguez Julien,
Lemoyne Pierre,
Giordanengo Philippe,
Olivier Chrystel,
Lasnier Jacques,
Mauffette Yves,
Vincent Charles
Publication year - 2015
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1111/eea.12353
Subject(s) - biology , leafhopper , stylet , penetration (warfare) , xylem , botany , horticulture , anatomy , hemiptera , operations research , engineering
Feeding behavior of three leafhopper species – E rythroneura vitis ( H arris), E rythroneura ziczac ( W alsh), and E rythroneura elegantula ( S ay) ( H emiptera: C icadellidae) – reared on grapevine, V itis vinifera L. cv. ‘Seyval blanc’ ( V itaceae), was investigated using histological techniques and DC ‐electrical penetration graphs ( DC ‐ EPG ). Histological studies revealed that the E rythroneura species induced white stipples on the leaves and that these leafhoppers produced thin salivary sheaths in grapevine leaf tissues. The DC ‐ EPG system allowed the characterization of five waveforms associated with stylet penetration and feeding in leaf tissues. These waveforms were characteristic of feeding phases corresponding to epidermis penetration pathway, salivation, and ingestion. We calculated 28 parameters (e.g., number of probes, duration of phases, and time spent in the various tissues) to describe and compare the feeding behavior of the E rythroneura species. We conclude that the three E rythroneura species are mainly mesophyll feeders but may probably also feed in other tissues such as xylem.