Premium
Effect of avulsion storage media on periodontal ligament fibroblast differentiation
Author(s) -
Bağ İrem,
Yildirim Sibel
Publication year - 2017
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/edt.12356
Subject(s) - periodontal fiber , rankl , fibroblast , osteoprotegerin , balanced salt solution , runx2 , chemistry , population , andrology , cellular differentiation , microbiology and biotechnology , receptor , dentistry , activator (genetics) , immunology , medicine , osteoblast , biology , in vitro , biochemistry , gene , environmental health , organic chemistry
Abstract Background/Aim An avulsed tooth must be stored in a solution which maintains periodontal ligament ( PDL ) cell viability. The aim of this study was to compare the effects of Hank's balanced salt solution ( HBSS ) and milk on the differentiation of PDL fibroblasts. Materials and methods Eighteen extracted third molars, (n = 3 for each group), were immersed in HBSS , milk, and Dulbecco's modified Eagle medium‐Ham's F12 ( DMEM ‐F12) at 4°C for 30‐ to 60 minutes or 12 hours. The growth dynamics of PDL fibroblasts were evaluated with cell proliferation graphics and population doubling time ( PDT ) values. Runt‐related transcription factor 2 ( RUNX 2), receptor activator of nuclear factor kappa‐B ligand ( RANKL ), and collagen type XII ( COL 12) expression were used to evaluate the differentiation of PDL fibroblasts. Results The percentage of cell numbers and PDT values of groups were statistically insignificant. In the HBSS groups, RUNX 2 expression increased showing a direction to osteogenic differentiation of PDL fibroblasts. In the DMEM ‐F12 groups, RANKL expression increased, indicating there was a tendency for osteoclastogenic differentiation. In the milk groups, RUNX 2 expression decreased while other markers were stable showing PDL fibroblasts could protect fibroblast identity. Conclusions In terms of protecting fibroblast identity and resistance to differentiation, milk was more effective than HBSS .