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Blood concentration of prilocaine and lidocaine after the use of topical anesthesia (Oraqix ® ) in lacerated wounds
Author(s) -
AlMusawi Ala,
Matar Kamal,
Kombian Samuel,
Andersson Lars
Publication year - 2016
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/edt.12294
Subject(s) - prilocaine , lidocaine , pharmacokinetics , anesthesia , medicine , plasma concentration , absorption (acoustics) , chemistry , surgery , pharmacology , physics , acoustics
Background/Aim Soft tissue injuries have been reported as being sutured using only topical anesthesia applied in the laceration wound. The objective of this study was to assess the pharmacokinetic profile of components of Oraqix ® (2.5% prilocaine and 2.5% lidocaine) when applied in a laceration as compared to intact skin application in the mouse. Materials and methods A total of 200 BALB/c male mice were used in this study. The mice were divided into three groups: group A: shaved and laceration group (80 mice); B: shaved and intact skin group (80 mice); and C: control group (shaved, no treatment; 40 mice) which underwent the same procedures but without application of Oraqix ® . Blood samples were collected over 90 min. Plasma sample analysis employing liquid chromatography coupled with the tandem mass spectrometric (LC‐MS/MS) method was used to determine plasma concentrations of lidocaine and prilocaine. Pharmacokinetic analysis of mouse plasma concentrations was carried out by standard non‐compartmental methods. Results Absorption of both lidocaine and prilocaine was rapid. C max and AUC values of lidocaine were significantly increased by fourfold and twofold, respectively, in lacerated mouse skin compared to intact skin. Similarly, prilocaine's C max and AUC values were also increased by 2.5‐fold and fourfold, respectively, in lacerated skin compared to intact skin. Conclusion When Oraqix ® was applied directly into the skin laceration, the plasma concentration of lidocaine and prilocaine was significantly increased as compared to when applied on intact skin. The present study, albeit in mice, indicates that the plasma levels of lidocaine and prilocaine can reach very high levels when the thermosetting gel Oraqix ® is placed directly in wounds.