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The effects of radicular dentine treated with double antibiotic paste and ethylenediaminetetraacetic acid on the attachment and proliferation of dental pulp stem cells
Author(s) -
Kim Ki Wan,
Yassen Ghaeth H.,
Ehrlich Ygal,
Spolnik Kenneth,
Platt Jeffrey A.,
Windsor L. Jack
Publication year - 2015
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/edt.12181
Subject(s) - ethylenediaminetetraacetic acid , dental pulp stem cells , incubation , chemistry , pulp (tooth) , dentistry , andrology , in vitro , medicine , biochemistry , chelation , organic chemistry
Aim This study explored the effects of dentine treated with two concentrations of double antibiotic paste ( DAP ) and ethylenediaminetetraacetic acid ( EDTA ) on the attachment and proliferation of dental pulp stem cells ( DPSC s). Materials and Methods Radicular dentine samples were prepared with identical dimensions and randomized into six groups ( n = 4). Four groups were treated with double antibiotic paste ( DAP ) at concentrations of 500 mg ml −1 or 1 mg ml −1 with or without EDTA . The other two groups were treated with EDTA only or received no treatment. DPSC s were seeded on each dentine sample (10 000 cells per sample). Lactate dehydrogenase activity assays were used to calculate the attached DPSC s after 1 day of incubation. Water soluble tetrazolium assays were performed to investigate DPSC s proliferation on the treated dentine samples after three additional days of incubation. Two‐way anova followed by Tukey–Kramer tests was used for statistical analyses (α = 0.05). Results Dentine treated with 1 or 500 mg ml −1 of DAP followed by EDTA caused significant increases in DPSC s attachment compared to the dentine treated with the DAP alone. The 500 mg ml −1 of DAP with or without EDTA caused significant reductions in DPSC s proliferation. However, the treatment of dentine with 1 mg ml −1 of DAP did not have significant negative effects on DPSC s proliferation regardless of the use of EDTA. Conclusion The use of 1 mg ml −1 of DAP followed by 10 min of irrigation with EDTA in endodontic regeneration procedure may have no negative effects on the attachment and proliferation of DPSC s.