Premium
Potential of coconut water and soy milk for use as storage media to preserve the viability of periodontal ligament cells: an in vitro study
Author(s) -
Moura Camilla Cristhian Gomes,
Soares Priscilla Barbosa Ferreira,
Paula Reis Manuella Verdinelli,
Fernandes Neto Alfredo Júlio,
Zanetta Barbosa Darceny,
Soares Carlos José
Publication year - 2014
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/edt.12042
Subject(s) - soy milk , viability assay , periodontal fiber , food science , shelf life , trypan blue , skimmed milk , chemistry , balanced salt solution , zoology , dentistry , biology , in vitro , medicine , biochemistry , organic chemistry
Aim There is no consensus regarding the ability of coconut water and soy milk to maintain long‐term cell viability. This study investigated the ability of pH ‐adjusted coconut water and soy milk to maintain the viability of periodontal ligament cells over a short and a longer period and compared these abilities with those of other solutions. Methods Dog premolar teeth were extracted, dried for 30 min, and stored in the following media for 50 min or 24 h: long shelf‐life whole milk (SWM), long shelf‐life skim milk (SSM), Hank's Balanced Salt Solution (HBSS), soy milk (SM), and pH ‐adjusted coconut water (CW). The positive and two negative control groups corresponded to 0‐min, 30‐min (short‐term), and 24‐h (long‐term) dry times, respectively. Cell viability was analyzed by trypan blue exclusion. Data were statistically analyzed using the Kruskal–Wallis test with post‐analysis using the Dunn method. Results In the short‐term experiment, the SSM resulted in significantly lower cell viability than SM and CW . At 24 h, SM and CW resulted in higher viability than HBSS and SSM and in comparable performance with the positive control group. Cell viability decreased over time, except in SM and CW . Conclusions Soy milk and pH ‐adjusted coconut water showed promising results as storage solutions for avulsed teeth, preserving the viability for up to 24 h.