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Expression of vasa , piwi , and nanos during gametogenesis in Typosyllis antoni (Annelida, Syllidae)
Author(s) -
PonzSegrelles Guillermo,
Bleidorn Christoph,
Aguado M. Teresa
Publication year - 2018
Publication title -
evolution and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 78
eISSN - 1525-142X
pISSN - 1520-541X
DOI - 10.1111/ede.12263
Subject(s) - biology , piwi interacting rna , gametogenesis , somatic cell , coelom , evolutionary biology , developmental biology , transcriptome , gamete , germ cell , stem cell , sperm , genetics , gene , gene expression , anatomy , genome , embryogenesis , transposable element
Although model species have proven to be crucial for developmental biology, the evo‐devo approach requires a broader picture across phylogeny. Herein, we try to expand the range of studied annelids by presenting a transcriptome of Typosyllis antoni as a tool for the study of developmental and evolutionary processes in Syllidae. Moreover, we provide homologs of the stem‐cell markers vasa , piwi , and nanos , and investigate their expression patterns in gamete‐producing individuals for the first time in this group. We found no expression in females, while there is a distinct expression pattern in males. Based on this data, we argue that spermatogenesis starts in the gonads and finishes in the coelomic cavity, and it occurs simultaneously in a large number of segments. Surprisingly, no expression of the stem‐cell markers was found in the segment addition zone of these reproducing animals (stolonizing). Preliminary explanations like a lack of growth during stolonization, or the absence of a common genetic program between germ and somatic stem cells, are discussed. Finally, no reservoir of primordial cells has been detected, suggesting a possible epigenic origin of the Primordial Germ Cells of this species, though this hypothesis needs to be further investigated.