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A precise review on NAATs ‐based diagnostic assays for COVID ‐19: A motion in fast POC molecular tests
Author(s) -
Maleki Bahareh,
Hojati Zohreh
Publication year - 2022
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.13853
Subject(s) - nucleic acid amplification tests , point of care testing , covid-19 , diagnostic test , medicine , loop mediated isothermal amplification , pandemic , infectious disease (medical specialty) , virology , disease , biology , pathology , emergency medicine , chlamydia trachomatis , dna , genetics
Background Diagnosis is one of the main strategies to deal with infectious and deadly diseases such as coronavirus disease 2019 (COVID‐19). The global pandemic of COVID‐19 has led to an immediate need to expand rapid diagnostic techniques. New isothermal‐based methods are being developed for COVID‐19 detection aiming to resolve the limitations related to the reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) method through immediate samples processing and minimizing false‐negative or ambiguous results. Advances in nucleic acid amplification techniques (NAATs) can provide affordable and easy‐to‐use diagnostic platforms with high sensitivity and specificity in order to be available to the public as approved commercial kits. Aims The development of point‐of‐care (POC) testing can assist in rapid clinical decision‐making and mitigate burdens on health care facilities. Finally, we discussed the different diagnostic methods based on NAATs for COVID‐19 in detail. Comparative parameters are addressed for all assays and Emergency Use Authorizations (EUA)‐approved commercial tests are cited. Conclusions Isothermal‐coupled methods and LAMP‐based molecular methods have been suggested as suitable portable tests with high diagnostic speed for use in POC testing.