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Age‐dependent messenger RNA expression of toll‐like receptor 4 and intercellular adhesion molecule‐1 in peripheral blood mononuclear cells
Author(s) -
Wen ChiungJung,
Chang ChinHao,
Chen ChinYing,
Peng JenKuei,
Huang HsienLiang,
Chuang PeiNi,
Chen ChingYu,
Tsai JawShiun
Publication year - 2021
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.13522
Subject(s) - peripheral blood mononuclear cell , tlr4 , immunology , tumor necrosis factor alpha , inflammation , messenger rna , medicine , toll like receptor , intercellular adhesion molecule 1 , receptor , real time polymerase chain reaction , biology , in vitro , gene , biochemistry , innate immune system
Background Inflammation plays an important role in the ageing process in which monocytes/macrophages are important players. Intercellular adhesion molecule‐1 (ICAM‐1), tumour necrosis factor‐α (TNF‐α) and Toll‐like receptor 4 (TLR4) are well‐known inflammatory markers. This study aimed to investigate the relationship between age and the expression and correlation of ICAM‐1, TNF‐α and TLR4 mRNA in peripheral blood mononuclear cells (PBMCs). Methods A total of 239 participants were recruited in a medical centre in Taiwan. The mRNA isolated from the PBMCs was used to determine the levels of ICAM‐1, TNF‐α and TLR4 mRNAs with real‐time polymerase chain reaction (PCR). The propensity‐matched analysis was also applied for subgroup analysis. Results When compared 189 older adults (≧65 years) to 50 younger adults (<65 years), the ICAM‐1, TNF‐α and TLR4 mRNA levels in PBMCs were significantly higher in older adults (2.00 ± 0.72 vs 0.87 ± 0.34 for ICAM‐1, 2.32 ± 0.69 vs 1.15 ± 0.44 for TNF‐α and 1.56 ± 0.47 vs 1.05 ± 0.51 for TLR4, and all P < .0001). Also, both age and TLR4 were independent factors affecting mononuclear cell ICAM‐1 in the multiple linear regression analysis ( P < .0001). Conclusion The mRNA levels of ICAM‐1 and TLR4 in PBMCs are higher in older adults than those in younger adults. TLR4 is an independent factor affecting ICAM‐1 expression in PBMCs, especially in older adults. This may suggest that ICAM‐1 and TLR4 in PBMCs are potential biomarkers and their relationship may shed some light on the ageing process.