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PET imaging during hypoglycaemia to study adipose tissue metabolism
Author(s) -
Boss Marti,
Rooijackers Hanne M. M.,
Buitinga Mijke,
Janssen Marcel J. R.,
Arens Anne I. J.,
GeusOei LioeFee,
Salm Liesbeth P.,
Galan Bastiaan E.,
Gotthardt Martin
Publication year - 2019
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.13120
Subject(s) - adipose tissue , type 2 diabetes , medicine , glucose uptake , endocrinology , diabetes mellitus , carbohydrate metabolism , glucose clamp technique , insulin , insulin resistance , pancreatic hormone
Background Disturbances in adipose tissue glucose uptake may play a role in the pathogenesis of type 2 diabetes, yet its examination by 2‐deoxy‐2‐[ 18 F]fluorodeoxyglucose ([ 18 F]FDG) PET/CT is challenged by relatively low uptake kinetics. We tested the hypothesis that performing [ 18 F]FDG PET/CT during a hypoglycaemic clamp would improve adipose tissue tracer uptake to allow specific comparison of adipose tissue glucose handling between people with or without type 2 diabetes. Design We enrolled participants with or without diabetes who were at least overweight, to undergo a hyperinsulinaemic hypoglycaemic clamp or a hyperinsulinaemic euglycaemic clamp (n = 5 per group). Tracer uptake was quantified using [ 18 F]FDG PET/CT. Results Hypoglycaemic clamping increased [ 18 F]FDG uptake in visceral adipose tissue of healthy participants ( P = 0.002). During hypoglycaemia, glucose uptake in visceral adipose tissue of type 2 diabetic participants was lower as compared to healthy participants ( P < 0.0005). No significant differences were observed in skeletal muscle, liver or pancreas. Conclusions The present findings indicate that [ 18 F]FDG PET/CT during a hypoglycaemic clamp provides a promising new research tool to evaluate adipose tissue glucose metabolism. Using this method, we observed a specific impairment in visceral adipose tissue [ 18 F]FDG uptake in type 2 diabetes, suggesting a previously underestimated role for adipose tissue glucose handling in type 2 diabetes.