PET imaging during hypoglycaemia to study adipose tissue metabolism

Background Disturbances in adipose tissue glucose uptake may play a role in the pathogenesis of type 2 diabetes, yet its examination by 2‐deoxy‐2‐[ 18 F]fluorodeoxyglucose ([ 18 F]FDG) PET/CT is challenged by relatively low uptake kinetics. We tested the hypothesis that performing [ 18 F]FDG PET/CT during a hypoglycaemic clamp would improve adipose tissue tracer uptake to allow specific comparison of adipose tissue glucose handling between people with or without type 2 diabetes. Design We enrolled participants with or without diabetes who were at least overweight, to undergo a hyperinsulinaemic hypoglycaemic clamp or a hyperinsulinaemic euglycaemic clamp (n = 5 per group). Tracer uptake was quantified using [ 18 F]FDG PET/CT. Results Hypoglycaemic clamping increased [ 18 F]FDG uptake in visceral adipose tissue of healthy participants ( P  = 0.002). During hypoglycaemia, glucose uptake in visceral adipose tissue of type 2 diabetic participants was lower as compared to healthy participants ( P  < 0.0005). No significant differences were observed in skeletal muscle, liver or pancreas. Conclusions The present findings indicate that [ 18 F]FDG PET/CT during a hypoglycaemic clamp provides a promising new research tool to evaluate adipose tissue glucose metabolism. Using this method, we observed a specific impairment in visceral adipose tissue [ 18 F]FDG uptake in type 2 diabetes, suggesting a previously underestimated role for adipose tissue glucose handling in type 2 diabetes.