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Peptidomic analysis of cartilage and subchondral bone in OA patients
Author(s) -
Gatenholm Birgitta,
Gobom Johan,
Skillbäck Tobias,
Blennow Kaj,
Zetterberg Henrik,
Brittberg Mats
Publication year - 2019
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.13082
Subject(s) - osteoarthritis , medicine , cartilage , tandem mass spectrometry , subchondral bone , tandem mass tag , articular cartilage , pathology , mass spectrometry , anatomy , chromatography , chemistry , proteomics , biochemistry , alternative medicine , quantitative proteomics , gene
Background The objective of this study was to develop a method for directly analysing osteochondral samples straight out of the operating room without cell culturing, thereby enabling identification of potential peptide biomarkers to better understand the mechanisms involved in the development of osteoarthritis and pain. Material and Methods Osteochondral plugs from wounded and macroscopically nonwounded zones of the femur condyle were collected from six patients with manifest osteoarthritis (OA) undergoing total knee arthroplasty (TKA). The samples were demineralized and supernatant was collected and isotopically marked with Tandem Mass Tag (TMT) labelling and analysed using liquid chromatography coupled with tandem mass spectrometry LC‐MS/MS. Results Using peptidomics, 6292 endogenous peptides were identified. Five hundred sixty‐six peptides (8 identified endogenous peptides) differed significantly ( P ‐value 0.10) from wounded zones compared to nonwounded zones. Conclusion This pilot study shows promising results for enabling peptidomic analysis of cartilage and bone straight out of the operating room. With further refinement, peptidomics can potentially become a diagnostic tool for OA, and improve the knowledge of disease progression and genesis of pain.