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A positive correlation exists between neurotrauma and TGF ‐β1‐containing microglia in rats
Author(s) -
Chio ChungChing,
Lin MaoTsun,
Chang ChingPing,
Lin HungJung
Publication year - 2016
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.12693
Subject(s) - microglia , traumatic brain injury , neuroprotection , transforming growth factor , medicine , correlation , endocrinology , pathology , inflammation , psychiatry , geometry , mathematics
Background Transforming growth factor‐beta 1 ( TGF ‐β1) regulates many processes after traumatic brain injury ( TBI ). Both Neuro AiD™ ( MLC 601) and astragaloside ( AST ) attenuate microglia activation in rats with TBI . The purpose of this study was to investigate whether MLC 601 or AST improves output of TBI by affecting microglial expression of TGF ‐β1. Materials and methods Adult male Sprague‐Dawley rats (120 in number) were used to investigate the contribution of TGF ‐β1‐containing microglia in the MLC 601‐mediated or the AST ‐mediated neuroprotection in the brain trauma condition using lateral fluid percussion injury. Results Pearson correlation analysis revealed that there was a positive correlation between brain injury (evidenced by both brain contused volume and neurological severity score) and the cortical numbers of TGF ‐β1‐containing microglia for the rats ( n = 12) 4 days post‐ TBI . MLC 601 or AST significantly ( P < 0·05) attenuated TBI ‐induced brain contused volume (119 ± 14 mm 3 or 108 ± 11 mm 3 vs. 160 ± 21 mm 3 ), neurological severity score (7·8 ± 0·3 or 8·1 ± 0·4 vs. 10·2 ± 0·5) and numbers of TGF ‐β1‐containing microglia (6% ± 2% or 11% ± 3% vs. 79% ± 7%) for the rats 4 days post‐ TBI . Conclusions There was a positive correlation between TBI and cortical numbers of TGF ‐β1‐containing microglia which could be significantly attenuated by astragaloside or NeuroAiD™ ( MLC 601) in rats.