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Development of a dietary‐induced metabolic syndrome model using miniature pigs involvement of AMPK and SIRT 1
Author(s) -
Li SinJin,
Liu ChiaHsin,
Chang CheiWei,
Chu HsienPin,
Chen KuenJaw,
Mersmann Harry J.,
Ding ShihTorng,
Chu ChunHan,
Chen ChingYi
Publication year - 2015
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.12370
Subject(s) - ampk , cls upper limits , triglyceride , metabolic syndrome , endocrinology , medicine , lipid metabolism , fatty liver , obesity , biology , cholesterol , disease , biochemistry , protein kinase a , kinase , optometry
Background During the progression of the metabolic syndrome (MetS), cardiovascular diseases ( CVD ) appear clinically in many individuals and cause death. As a result, it is essential to set up an optimal animal model to study the mechanism of MetS leading to CVD . SIRT 1 and AMPK are the master regulators of lipid and carbohydrate metabolism. The objective of this study was to establish a miniature pig model of Western diet‐induced MetS and investigate the role of SIRT 1/ AMPK during MetS development. Materials and Methods Five‐month‐old Lee‐Sung ( LS ) and Lanyu ( LY ) minipigs were each randomly assigned to two groups: control diet (C) and Western diet (W), in a 6‐month experimental period. Results Western diet caused obesity in both minipig models. Compared with the CLS pigs, WLS pigs exhibited hypercholesterolaemia. However, WLY pigs maintained a similar plasma lipid profile to the CLY pigs. Western diet caused a lower antioxidant capacity in the liver of both pig models. WLS pigs had higher triglyceride accumulation in the liver than CLS pigs, whereas WLY and CLY pigs had similar hepatic triglyceride accumulation. Compared with CLS pigs, WLS pigs had a lower hepatic SIRT 1 expression, whereas WLY pigs had a higher expression of AMPK , FOXO 1 and SIRT 1 than CLY pigs. Conclusion Long‐term feeding of the Western diet to Lee‐Sung miniature pigs not only caused obesity but also induced MetS and fatty liver, whereas Western diet induced obesity in Lanyu pigs without metabolic dysfunctions. SIRT 1/ AMPK and their downstream pathways might be one of the possible regulators for pathological obesity in Lee‐Sung pigs.