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Molecular profiling of EGFR family in chronic obstructive pulmonary disease: correlation with airway obstruction
Author(s) -
Anagnostis Aristotelis,
Neofytou Eirini,
Soulitzis Nikolaos,
Kampas Dimitris,
Drositis Ioannis,
Dermitzaki Despina,
Tzanakis Nikolaos,
Schiza Sofia,
Siafakas Nikolaos M.,
Tzortzaki Eleni G.
Publication year - 2013
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.12178
Subject(s) - copd , medicine , amphiregulin , lung , epidermal growth factor receptor , erbb , mucociliary clearance , pathology , receptor , gastroenterology
Background Growth factors mediate various cellular responses to environmental stimuli. Specifically, exposure of lung epithelium to oxidative stress induced by cigarette smoke stimulates aberrant epidermal growth factor receptor ( ERBB ) family activation. This study's objective was to evaluate the expression of ERBB 1–4 receptors in the lung tissue of smokers with or without chronic obstructive pulmonary disease ( COPD ). Materials and methods ERBBs expression was measured by microarray analysis in lung tissue samples from five patients with COPD and five non‐ COPD smokers, and by quantitative real‐time PCR in additional 20 patients with COPD ( GOLD stage II ), 15 non‐ COPD smokers and 10 nonsmoker controls. Results Microarray data analysis revealed that ERBB receptors expression was elevated in patients with COPD compared to non‐ COPD smokers, ranging from 1·62‐ to 2·45‐fold, ( P < 0·01). Real‐time qPCR verified that patients with COPD had higher ERBB 1–3 expression levels compared with non‐ COPD smokers ( P ERBB1 < 0·001; P ERBB2 = 0·003; P ERBB3 = 0·003) and nonsmokers ( P ERBB1 = 0·019; P ERBB2 = 0·005; P ERBB3 = 0·011). On the other hand, ERBB 4 mRNA levels gradually increased from nonsmokers (0·74 ± 0·19) to non‐ COPD smokers (1·11 ± 0·05) to patients with COPD (1·57 ± 0·28) and were correlated with the degree of airflow obstruction ( P FEV1 < 0·001). Discussion These data suggest that ERBB 1–3 overexpression is not related only to smoking exposure but probably to epithelial remodelling and mucociliary system distortion, characterizing COPD . Additionally, the inverse correlation of ERBB 4 with FEV 1 exhibits a possible link between ERBB 4 and COPD severity.