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The LPS ‐induced increase in circulating microparticles is not affected by vitamin C in humans
Author(s) -
Weisshaar Stefan,
Gouya Ghazaleh,
Nguyen Daniel,
Kapiotis Stylianos,
Wolzt Michael
Publication year - 2013
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.12096
Subject(s) - inflammation , vitamin , saline , in vivo , chemistry , medicine , endocrinology , microparticle , c reactive protein , systemic inflammation , vitamin d and neurology , immunology , biology , microbiology and biotechnology , astrobiology
Objective Microparticles ( MP ) are considered to promote coagulation. This study aimed to characterize the time course of MP levels and the effect of high‐dose vitamin C on MP formation during inflammation in an in vivo E scherichia coli endotoxin ( LPS ) model. Methods Microparticle formation was studied in 14 male subjects in a cross‐over trial who received either intravenous vitamin C at 320 mg/kg body weight ( BW ) or 480 mg/kg BW or saline solution in a random order on alternate trial days 3 h after intravenous exposure to LPS (2 ng/kg BW). Venous blood samples were taken before, 3 and 6 h after LPS . D‐dimer, leucocyte count, C‐reactive protein, plasma vitamin C and body temperature were assessed as inflammatory parameters. MP were detected using flow cytometric analysis and expressed in 10³ MP/mL plasma. Results Microparticles levels were decreased from baseline 848 units [range 431–1705] by 21% to 671 units [253–1586] at 3 h and increased by 32% to 1119 units [288–4443] at 6 h after LPS . This pattern was not influenced by administration of vitamin C, with a change from 730 units [399–1396] at baseline by an increase to 832 units [215–2168] at 3 h to 1055 units [350–4858] at 6 h. MP subpopulations followed similar dynamics. Alterations in inflammatory parameters were independent from vitamin C administration during endotoxemia. Conclusion Microparticles are increased in acute systemic inflammation with inconsistent changes in MP subgroups in healthy subjects. Systemic vitamin C administration does not mitigate MP formation and D‐dimer levels during acute systemic inflammation, suggesting that MP ‐induced coagulation activity is not affected by vitamin C.