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Cinacalcet effect on polymorphonuclear leucocytes of kidney transplant patients
Author(s) -
Cohen Gerald,
Raupachova Jana,
Borchhardt Kyra,
Hörl Walter H.
Publication year - 2013
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/eci.12075
Subject(s) - cinacalcet , hypercalcaemia , calcium , kidney , endocrinology , phagocytosis , medicine , respiratory burst , kidney transplantation , calcium in biology , immunology , chemistry , secondary hyperparathyroidism , parathyroid hormone
Background Polymorphonuclear leucocytes ( PMNL s) play a key role in the nonspecific immune defence. Cinacalcet reduces serum calcium levels in kidney transplant recipients with mineral bone disorder associated with chronic kidney disease. We investigated essential functions of PMNL s of kidney transplant recipients with and without hypercalcaemia and with and without cinacalcet therapy. Subjects and methods Oxidative burst, phagocytosis, apoptosis and intracellular calcium concentrations of PMNL s from normocalcaemic kidney transplant patients without ( KT ‐ NC ) or with cinacalcet intake ( KT ‐ NC / CI ), hypercalcaemic kidney transplant patients ( KT ‐ HC ) and healthy subjects ( HS ) were investigated. Results Stimulation of oxidative burst of PMNL s from KT ‐ HC patients by phorbol‐12‐myristate‐13‐acetate or E scherichia coli was significantly attenuated compared with PMNL s from KT ‐ NC , KT ‐ NC / CI and HS . Apoptosis of PMNL s from KT ‐ HC patients was significantly decreased compared with cells from KT ‐ NC , KT ‐ NC / CI and HS . Apoptosis correlated significantly with serum calcium concentrations. Intracellular calcium concentrations and phagocytosis of PMNL s did not differ between groups. Conclusions Our data indicate that stimulation of PMNL oxidative burst and apoptosis is significantly diminished in kidney transplant patients with hypercalcaemia, while kidney transplant patients with serum calcium levels normalized by cinacalcet have normal PMNL functions despite immunosuppressive therapy.