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A 4D view on insulin secretory granule turnover in the β‐cell
Author(s) -
Müller Andreas,
Mziaut Hassan,
Neukam Martin,
Knoch KlausPeter,
Solimena Michele
Publication year - 2017
Publication title -
diabetes, obesity and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.445
H-Index - 128
eISSN - 1463-1326
pISSN - 1462-8902
DOI - 10.1111/dom.13015
Subject(s) - granule (geology) , insulin , secretion , intracellular , microbiology and biotechnology , ultrastructure , cytoskeleton , cell , biology , chemistry , medicine , endocrinology , biochemistry , anatomy , paleontology
Insulin secretory granule (SG) turnover consists of several highly regulated processes allowing for proper β‐cell function and insulin secretion. Besides the spatial distribution of insulin SGs, their age has great impact on the likelihood of their secretion and their behaviour within the β‐cell. While quantitative measurements performed decades ago demonstrated the preferential secretion of young insulin, new experimental approaches aim to investigate insulin ageing at the granular level. Live‐cell imaging, automated image analysis and correlative light and electron microscopy have fostered knowledge of age‐defined insulin SG dynamics, their interaction with the cytoskeleton and ultrastructural features. Here, we review our recent work in regards to the connection between insulin SG age, SG dynamics, intracellular location and interaction with other proteins.