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Efficiently accumulating germ‐like stem cells from mouse postnatal ovary by in situ tissue culture
Author(s) -
Zhang Meizi,
Liu Li,
Cao Xiaomin,
Liu Ye,
Di Jianyong,
Huang Xiuying,
Sun Fangzhen,
Huang Weihong,
Xu Fengqin
Publication year - 2020
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12656
Subject(s) - biology , germline , ovary , germ cell , stem cell , microbiology and biotechnology , oogenesis , developmental biology , germ , germ line development , cell culture , genetics , oocyte , embryo , gene
Although recent studies have revealed that germline stem cells (GSCs) exist in the mouse postnatal ovary, how to efficiently obtain GSCs for regenerating neo‐oogenesis is still a technical challenge. Here, we report that using in situ tissue culture we can efficiently accumulate large amounts of proliferating germ‐like cells from mouse postnatal ovaries. Usually, more than 10,000 germ‐like cells can be derived from one ovary by this method, and over 20% of these cells can grow into germ‐like cells with self‐renewal, which thus can serve as a good cell pool to isolate GSCs by other cell assorting methods such as FACS. This method is simple and time‐saving, which should be useful for in future studies on mouse GSCs.