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Akhirin regulates the proliferation and differentiation of neural stem cells/progenitor cells at neurogenic niches in mouse brain
Author(s) -
Anam Mohammad Badrul,
Ahmad Shah Adil Ishtiyaq,
Kudo Mikiko,
Istiaq Arif,
Felemban Athary Abdulhaleem M.,
Ito Naofumi,
Ohta Kunimasa
Publication year - 2020
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12646
Subject(s) - neurogenesis , neural stem cell , subventricular zone , dentate gyrus , biology , progenitor cell , neurosphere , embryonic stem cell , stem cell , neuroscience , microbiology and biotechnology , hippocampal formation , hippocampus , adult stem cell , genetics , gene
Abstract Specialized microenvironment, or neurogenic niche, in embryonic and postnatal mouse brain plays critical roles during neurogenesis throughout adulthood. The subventricular zone (SVZ) and the dentate gyrus (DG) of hippocampus in the mouse brain are two major neurogenic niches where neurogenesis is directed by numerous regulatory factors. Now, we report Akhirin (AKH), a stem cell maintenance factor in mouse spinal cord, plays a pivotal regulatory role in the SVZ and in the DG. AKH showed specific distribution during development in embryonic and postnatal neurogenic niches. Loss of AKH led to abnormal development of the ventricular zone and the DG along with reduction of cellular proliferation in both regions. In AKH knockout mice (AKH −/− ), quiescent neural stem cells (NSCs) increased, while proliferative NSCs or neural progenitor cells decreased at both neurogenic niches. In vitro NSC culture assay showed increased number of neurospheres and reduced neurogenesis in AKH −/− . These results indicate that AKH, at the neurogenic niche, exerts dynamic regulatory role on NSC self‐renewal, proliferation and differentiation during SVZ and hippocampal neurogenesis.