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Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus
Author(s) -
Ishimaru Yoshiyasu,
Bando Tetsuya,
Ohuchi Hideyo,
Noji Sumihare,
Mito Taro
Publication year - 2018
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12560
Subject(s) - gryllus bimaculatus , biology , gene knockdown , regeneration (biology) , decapentaplegic , rna interference , microbiology and biotechnology , tarsus (eyelids) , anatomy , bone morphogenetic protein 2 , rna , imaginal disc , genetics , phenotype , cricket , gene , medicine , zoology , eyelid , surgery , in vitro
The cricket, Gryllus bimaculatus , is a classic model of leg regeneration following amputation. We previously demonstrated that Gryllus decapentaplegic ( Gb ’ dpp ) is expressed during leg regeneration, although it remains unclear whether it is essential for this process. In this study, double‐stranded RNA targeting the Smad mathers‐against‐dpp homolog, Gb ’ mad , was used to examine the role of bone morphogenetic protein ( BMP ) signaling in the leg regeneration process of Gryllus bimaculatus . RNA interference ( RNA i)‐mediated knockdown of Gb ’ mad led to a loss of tarsus regeneration at the most distal region of regenerating leg segments. Moreover, we confirmed that the phenotype obtained by knockdown of Dpp type I receptor, Thick veins ( Gb ’ tkv ), closely resembled that observed for Gb ’ mad RNA i crickets, thereby suggesting that the BMP signaling pathway is indispensable for the initial stages of tarsus formation. Interestingly, knockdown of Gb ’ mad and Gb ’ tkv resulted in significant elongation of regenerating tibia along the proximodistal axis compared with normal legs. Moreover, our findings indicate that during the regeneration of tibia, the BMP signaling pathway interacts with Dachsous/Fat ( Gb ’Ds/ Gb ’Ft) signaling and dachshund ( Gb ’ dac ) to re‐establish positional information and regulate determination of leg size. Based on these observations, we discuss possible roles for Gb ’ mad in the distal patterning and intercalation processes during leg regeneration in Gryllus bimaculatus .