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High mobility group box transcription factor 1 (HBP1) from Lampetra japonica affects cell cycle regulation
Author(s) -
Song Xiaoping,
Gao Xingxing,
Lu Jiali,
Liang Hongfang,
Su Peng,
Li Qingwei,
Pang Yue
Publication year - 2018
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12426
Subject(s) - biology , cell cycle , lamprey , microbiology and biotechnology , transfection , gene , genetics , fishery
Abstract High mobility group ( HMG ) box‐containing protein 1 ( HBP 1) is a member of the HMG family of chromosomal proteins. Previous studies have shown that human HBP 1 exhibits tumor‐suppressor activity. Here, we identified a homologue of HBP 1, L‐hbp1 , in Lampetra japonica . The L‐hbp1 gene shared high sequence similarity with its homologues in jawed vertebrates, as shown by bioinformatics analyses. L‐hbp1 contains a 1,584‐bp open reading frame that encodes 527 amino acids. A pA denox‐L‐ HBP 1 plasmid was constructed and transfected successfully in Raji cells, as revealed by real‐time PCR . The overexpression of L‐ HBP 1 reduced cell growth rates, inhibited G1 phase progression, decreased cyclin D1 and c‐Myc protein expression, and increased p53 protein expression. Western blot and immunohistochemical assays showed that L‐ HBP 1 was primarily distributed in the heart, kidney, gill and liver of lamprey. Cell cycle analysis revealed that decreased L‐ HBP 1 expression in HBP 1 morpholino oligonucleotide‐transfected lamprey cells resulted in a decreased fraction of cells in the G1 phase and corresponding increases in the S and G2/M phases. Additionally, treatment of lamprey cardiac cells with pharmacological inhibitors of p38 MAP kinase released the cells from G1 arrest. Together, these results indicated that HBP 1 expression in lamprey was correlated with the onset of mitotic arrest in these cells, which have implications for cell cycle regulation.