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Ebp1 regulates myogenic differentiation of myoblast cells via SMAD 2/3 signaling pathway
Author(s) -
Yu Minli,
Wang Huan,
Liu Zhen,
Lu Yinglin,
Yu Debing,
Li Dongfeng,
Du Wenxing
Publication year - 2017
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12380
Subject(s) - myogenesis , myod , myocyte , myogenin , c2c12 , myf5 , gene knockdown , cellular differentiation , microbiology and biotechnology , biology , downregulation and upregulation , myogenic regulatory factors , multinucleate , signal transduction , cell culture , biochemistry , genetics , gene
Regulation of skeletal muscle development requires many of the regulatory networks that are fundamental to developmental myogenesis. ErbB3 binding protein‐1 ( Ebp1 ) is involved in the control of myoblasts development in chicken. However, the expression and biological functions of Ebp1 in the progress of myogenesis are unclear. This study focused on determining the effect of Ebp1 on myogenic proliferation and differentiation using a primary myoblasts culture model. Ebp1 was found to upregulate in proliferating myoblasts and decrease at the early stage of myogenic differentiation. The level of endogenous Ebp1 increased from E9 to E20 chicken leg muscles. Knockdown of Ebp1 had no effect on myoblasts proliferation. However, myogenic differentiation into multinucleated myotubes was significantly reduced. The mRNA and protein expression of MRF s was decreased when Ebp1 was knocked down. Downregulation of Ebp1 , accompanied by elevated levels of pSMAD 2/3, suggests that Ebp1 is involved in regulating myogenic differentiation via SMAD 2/3 inhibition. The phosphorylation of SMAD 2/3 was activated and the expression of MYOD and MYOG was reduced in Ebp1 knockdown myoblasts, but addition of LY 2109761 (an inhibitor specified to SMAD 2/3) blocked these effects. Collectively, these results indicate that Ebp1 promotes myoblast differentiation by inhibition of SMAD 2/3 signaling pathway during chicken myogenesis. These data provide new insights into the biological role of Ebp1 in embryonic chicken skeletal muscle development.