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Live imaging of X chromosome inactivation and reactivation dynamics
Author(s) -
Kobayashi Shin
Publication year - 2017
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12365
Subject(s) - epigenetics , x inactivation , live cell imaging , biology , stem cell , developmental biology , genetics , x chromosome , chromosome , microbiology and biotechnology , gene , evolutionary biology , cell
The epigenetic phenomenon called X chromosome inactivation plays critical roles in female development in eutherian mammals, and has attracted attention in the fields of developmental biology and regenerative biology in efforts to understand the pluripotency of stem cells. X chromosome inactivation is routinely studied after cell fixation, but live imaging is increasingly being required to improve our understanding of the dynamics and kinetics of X chromosome inactivation and reactivation processes. Here, we describe our live imaging method to monitor the epigenetic status of X chromosomes using a gene knock‐in mouse strain named “Momiji” and give an overview of the application of this strain as a resource for biological and stem cell research.