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Human gingival fibroblasts induced and differentiated into vascular endothelial‐like cells
Author(s) -
Liu Xuqian,
Wang Jie,
Dong Fusheng,
Li Hexiang,
Hou Yali
Publication year - 2016
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12327
Subject(s) - cd31 , biology , flow cytometry , vimentin , cd34 , endothelial stem cell , microbiology and biotechnology , fibroblast , pathology , connective tissue , stem cell , cell culture , in vitro , immunology , immunohistochemistry , medicine , biochemistry , genetics
A novel method for repair of vascular disease, mechanical damage, and tissue rebuilding is urgently required. Vascular endothelial cells ( VEC s) play an essential role in vascular rebuilding and vasotransplantation. In the present study, human gingival fibroblasts ( HGF s) were cultured and induced into endothelial‐like cells in vitro in order to confirm that HGF s with stem cell properties possessed the potential for differentiation into endothelial‐like cells. The epithelium was extracted from normal human gingiva consisting of epithelium and connective tissue, which was isolated from patients. The identification of HGF s and induced endothelial‐like cells were confirmed by flow cytometry, reverse transcription polymerase chain reaction ( RT – PCR ), immunocytochemical stain ( ICS ), and immunofluorescence stain ( ISA ). The morphology of human gingival fibroblasts with 8 ng/mL VEGF 165 induced for different periods of days were observed by inverted microscope. Before induction, flow cytometry analysis showed that HGF s were positive for vimentin, but negative for CD 31. RT – PCR , ICS , and ISA showed vimentin, S100A4, α ‐ SMA , collagen III , and S100A4 were specifically expressed in these fibroblast cells. After induction, ICS showed induced vascular endothelial‐like cells were positive for CD 34 and CD 31; ISA showed cells induced were positive for vWF and E‐cadherin; RT – PCR results demonstrated that tie2 was specifically expressed in the cells induced. Flow cytometry analysis of the transformation efficiency from HGF s to endothelial‐like cells. In conclusion, we found that HGF s possessed capacity for being induced and differentiated into vessel endothelial‐like cells with typical and specific morphological, ultrastructural, and immunological characters of endothelial‐like cells by induction with VEGF .