Premium
Adenosine Triphosphate stimulates differentiation and mineralization in human osteoblast‐like Saos‐2 cells
Author(s) -
Cutarelli Alessandro,
Marini Mario,
Tancredi Virginia,
D'Arcangelo Giovanna,
Murdocca Michela,
Frank Claudio,
Tarantino Umberto
Publication year - 2016
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12288
Subject(s) - runx2 , alkaline phosphatase , osteoblast , mineralization (soil science) , osteocalcin , microbiology and biotechnology , purinergic receptor , chemistry , cellular differentiation , cell growth , adenosine triphosphate , receptor , adenosine , purinergic signalling , medicine , endocrinology , adenosine receptor , biology , biochemistry , in vitro , enzyme , gene , agonist , organic chemistry , nitrogen
In the last years adenosine triphosphate (ATP) and subsequent purinergic system activation through P2 receptors were investigated highlighting their pivotal role in bone tissue biology. In osteoblasts ATP can regulate several activities like cell proliferation, cell death, cell differentiation and matrix mineralization. Since controversial results exist, in this study we analyzed the ATP effects on differentiation and mineralization in human osteoblast‐like Saos‐2 cells. We showed for the first time the altered functional activity of ATP receptors. Despite that, we found that ATP can reduce cell proliferation and stimulate osteogenic differentiation mainly in the early stages of in vitro maturation as evidenced by the enhanced expression of alkaline phosphatase (ALP), Runt‐related transcription factor 2 (Runx2) and Osteocalcin (OC) genes and by the increased ALP activity. Moreover, we found that ATP can affect mineralization in a biphasic manner, at low concentrations ATP always increases mineral deposition while at high concentrations it always reduces mineral deposition. In conclusion, we show the osteogenic effect of ATP on both early and late stage activities like differentiation and mineralization, for the first time in human osteoblastic cells.