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Knockdown of zebrafish Nanog increases primordial germ cells during early embryonic development
Author(s) -
Wang Huannan,
Liu Yanhua,
Ye Ding,
Li Jianzhen,
Liu Jiangdong,
Deng Fengjiao
Publication year - 2016
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12279
Subject(s) - homeobox protein nanog , biology , zebrafish , gene knockdown , embryonic stem cell , microbiology and biotechnology , rex1 , embryogenesis , epiboly , embryo , gene , genetics , gastrulation , adult stem cell , induced pluripotent stem cell
Nanog is a homeodomain transcription factor that plays a prominent role in maintaining the pluripotency and self‐renewal capacity of embryonic stem cells ( ESC s) in mammals. Medaka Nanog is necessary for S‐phase transition and proliferation during embryonic development. However, whether Nanog regulates the proliferation of primordial germ cells ( PGC s) during embryonic development has not yet been investigated. In this study, we identified the homologue of the mammalian Nanog gene in zebrafish ( zN anog ). The expression of both zN anog mRNA and protein was demonstrated in the spermatogonia (male germ stem cells) of the testis and the early oocytes of the ovary. During the embryonic development, zN anog mRNA is expressed in the cytoplasm of PGC s, and its protein is localized to the PGC nuclei. We also found that zN anog depletion using morpholinos resulted in the increases and aberrant localization of PGC s in the zebrafish embryos from the sphere stage to the 50% epiboly stage. These data indicated that zN anog inhibits the PGC s proliferation in early embryonic development of zebrafish.