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Heterogeneity of chromatoid bodies in adult pluripotent stem cells of planarian D ugesia japonica
Author(s) -
Kashima Makoto,
Kumagai Nobuyoshi,
Agata Kiyokazu,
Shibata Norito
Publication year - 2016
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12268
Subject(s) - planarian , induced pluripotent stem cell , microbiology and biotechnology , biology , regeneration (biology) , embryonic stem cell , biochemistry , gene
The robust regenerative ability of planarians is known to be dependent on adult pluripotent stem cells called neoblasts. One of the morphological features of neoblasts is cytoplasmic ribonucleoprotein granules (chromatoid bodies: CB s), which resemble germ granules present in germline cells in other animals. Previously, we showed by immuno‐electron microscopic analysis that Dj CBC ‐1, a planarian Me31B/Dhh1/ DDX 6 homologue, which is a component of ribonucleoprotein granules, was localized in CB s in the planarian Dugesia japonica . Also, recently it was reported using another planarian species that Y12 antibody recognizing symmetrical dimethylarginine ( sDMA ) specifically binds to CB s in which histone mRNA is co‐localized. Here, we showed by double immunostaining and RNA interference ( RNA i) that Dj CBC ‐1‐containing CB s and Y12‐immunoreactive CB s are distinct structures, suggesting that CB s are composed of heterogeneous populations. We also found that the Y12‐immunoreactive CB s specifically contained a cytoplasmic type of planarian PIWI protein (DjPiwiC). We revealed by RNA i experiments that Y12‐immunoreactive CB s may have anti‐transposable element activity involving the DjPiwiC protein in the neoblasts.