Premium
Comparison of fetal cartilage‐derived progenitor cells isolated at different developmental stages in a rat model
Author(s) -
Kim Mijin,
Kim Jiyoung,
Park So Ra,
Park Do Young,
Kim Young Jick,
Choi Byung Hyune,
Min ByoungHyun
Publication year - 2016
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12267
Subject(s) - chondrogenesis , sox2 , sox9 , cartilage , progenitor cell , microbiology and biotechnology , biology , adipogenesis , cellular differentiation , induced pluripotent stem cell , homeobox protein nanog , embryonic stem cell , runx2 , stem cell , mesenchymal stem cell , anatomy , transcription factor , genetics , gene
Fetal cartilage‐derived progenitor cells ( FCPC s) could be a useful cell source in cell‐based therapies for cartilage disorders. However, their characteristics can vary depending on the developmental stages. The aim of this study was to compare the characteristics of rat FCPC s from the hind limb on embryonic day 14 (E14), E16 and E20 regarding proliferation, pluripotency, and differentiation. Morphologically, rat fetal cartilage tissue showed an increase in cartilaginous differentiation features (Safranin‐O, type II collagen) and decrease in pluripotency marker (Sox2) in the order of E14, E16 and E20. E14 FCPC s showed significantly higher doubling time compared to E16 and E20 FCPC s. While the E14 FCPC s expressed pluripotent genes ( Sox2 , Oct4 , Nanog ), the E16 and E20 FCPC s expressed chondrogenic markers ( Sox9, Col2a1 , Acan ). E20 FCPC s showed the highest ability to both chondrogenic and adipogenic differentiation and E14 FCPC s showed relatively better activity in osteogenic differentiation. Further analysis showed that E20 FCPC s expressed both adipogenic ( C/ebpß ) and osteogenic ( Runx2, Sp7, Taz ) transcription factors as well as chondrogenic transcription factors. Our results show an inverse relationship overall between the expression of pluripotency genes and that of chondrogenic and lineage‐specific genes in FCPC s under development. Due to its exceptional proliferation and chondrogenic differentiation ability, fetal cells from epiphyseal cartilage (E20 in rats) may be a suitable cell source for cartilage regeneration.