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Blastocysts derivation from somatic cell fusion with premature oocytes (prematuration somatic cell fusion)
Author(s) -
Saadeldin Islam M.,
Khoirinaya Candrani,
Kim Su Jin,
Moon Joon Ho,
Almadaly Essam,
Lee Byeong Chun
Publication year - 2016
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12264
Subject(s) - somatic cell , somatic cell nuclear transfer , blastocyst , biology , inner cell mass , polar body , embryo , andrology , cell fusion , ploidy , microbiology and biotechnology , aneuploidy , genetics , oocyte , cell , embryogenesis , chromosome , gene , medicine
This study was undertaken to investigate the development of immature oocytes after their fusion with male somatic cells expressing red fluorescence protein ( RFP ). RFP ‐expressing cells were fused with immature oocytes, matured in vitro and then parthenogenetically activated. Somatic nuclei showed spindle formation, 1st polar body extrusion after in vitro maturation and protruded the 2nd polar body after parthenogenetic activation. RFP was expressed in the resultant embryos; two‐cell stage and blastocysts. Chromosomal analysis showed aneuploidy in 81.82% of the resulting blastocysts while 18.18% of the resulting blastocysts were diploid. Among eight RFP ‐expressing blastocysts, Xist mRNA s was detected in six while Sry mRNA was detected in only one blastocyst. We propose “prematuration somatic cell fusion” as an approach to generate embryos using somatic cells instead of spermatozoa. The current approach, if improved, would assist production of embryos for couples where the male partner is sterile, however, genetic and chromosomal analysis of the resultant embryos are required before transfer to the mothers.