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Analysis of cell growth and gene expression of porcine adipose tissue‐derived mesenchymal stem cells as nuclear donor cell
Author(s) -
Oh Hyun Ju,
Park Jung Eun,
Park Eun Jung,
Kim Min Jung,
Kim Geon A,
Rhee Sang Ho,
Lim Sang Hyun,
Kang Sung Keun,
Lee Byeong Chun
Publication year - 2014
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12159
Subject(s) - somatic cell nuclear transfer , sox2 , mesenchymal stem cell , biology , stem cell , adipose tissue , homeobox protein nanog , reprogramming , blastocyst , microbiology and biotechnology , andrology , cellular differentiation , gene expression , cell , embryo , embryonic stem cell , gene , embryogenesis , genetics , induced pluripotent stem cell , endocrinology , medicine
In several laboratory animals and humans, adipose tissue‐derived mesenchymal stem cells ( ASC ) are of considerable interest because they are easy to harvest and can generate a huge proliferation of cells from a small quantity of fat. In this study, we investigated: (i) the expression patterns of reprogramming‐related genes in porcine ASC ; and (ii) whether ASC can be a suitable donor cell type for generating cloned pigs. For these experiments, ASC , adult skin fibroblasts ( AF ) and fetal fibroblasts ( FF ) were derived from a 4‐year‐old female miniature pig. The ASC expressed cell‐surface markers characteristic of stem cells, and underwent in vitro differentiation when exposed to specific differentiation‐inducing conditions. Expression of DNA methyltransferase ( DNMT )1 in ASC was similar to that in AF , but the highest expression of the DNMT3B gene was observed in ASC . The expression of OCT4 was significantly higher in FF and ASC than in AF ( P < 0.05), and SOX2 showed significantly higher expression in ASC than in the other two cell types ( P < 0.05). After somatic cell nuclear transfer ( SCNT ), the development rate of cloned embryos derived from ASC was comparable to the development of those derived using FF . Total cell numbers of blastocysts derived using ASC and FF were significantly higher than in embryos made with AF . The results demonstrated that ASC used for SCNT have a potential comparable to those of AF and FF in terms of embryo in vitro development and blastocyst formation.