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Characterization of mesenchymal stem cells under the stimulation of Toll‐like receptor agonists
Author(s) -
Chen Xi,
Zhang ZhengYun,
Zhou Hao,
Zhou GuangWen
Publication year - 2014
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12124
Subject(s) - mesenchymal stem cell , immunogenicity , toll like receptor , immune system , microbiology and biotechnology , receptor , stimulation , inflammation , biology , chemistry , immunology , innate immune system , biochemistry , endocrinology
Infective factors cause the perpetuation of inflammation as a result of the permanent exposure of the immune system to exogenous or endogenous products of virus or bacteria. Mesenchymal stem cells ( MSC s) can be exposed to this infective environment, which may change the characteristics and therapeutic potency of these MSC s. MSC s have the ability to repair damaged and inflamed tissues and regulate immune responses. In this study, we demonstrated that MSC s express functional Toll‐like receptors ( TLR ) 3 and 4, the Toll‐like receptor families that recognize the signals of viral and bacterial mimics, respectively. The specific stimulations did not affect the self‐renewal and apoptosis capabilities of MSC s but instead promoted their differentiation into the adipocytes and osteoblasts with the TLR 3 ligand. The reverse of these results were obtained with the TLR 4 ligand. The migration of the MSC s to stimulate either of the two specific ligands was inhibited at different times, whereas the immunogenicity and immunosuppressive properties of the MSC s were not weakened unlike in the MSC s group. These results suggest that TLR 3 and TLR 4 stimulation affect the characterization of MSC s.