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Retracted: Effectiveness of cationic liposome‐mediated local delivery of myostatin‐targeting small interfering RNA in vivo
Author(s) -
Mori Hiroyo,
Kawai Nobuhiko,
Kinouchi Nao,
Hichijo Natsuko,
Ishida Tatsuhiro,
Kawakami Emi,
Noji Sumihare,
Tanaka Eiji
Publication year - 2014
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12123
Subject(s) - chemistry , small interfering rna , myostatin , myod , rna , downregulation and upregulation , cationic liposome , masseter muscle , duchenne muscular dystrophy , microbiology and biotechnology , transfection , medicine , endocrinology , biology , gene expression , biochemistry , anatomy , gene
This study evaluated the effectiveness of local administration of cationic liposome‐delivered myostatin‐targeting si RNA . Myostatin (Mst)‐si RNA and scrambled (scr)‐si RNA ‐lipoplexes were injected into the masseter muscles of wild type and dystrophin‐deficient mdx mice, which model Duchenne muscular dystrophy. One week after injection, the masseter muscles were dissected for histometric analyses. To evaluate changes in masseter muscle activity, masseter electromyographic ( EMG ) measurements were performed. One week after local administration of Mst‐si RNA ‐lipoplexes, masseter muscles and myofibrils were significantly larger compared to control masseter muscles treated with scr‐si RNA ‐lipoplexes. Real‐time polymerase chain reaction ( PCR ) analyses revealed significant upregulation of the myogenic regulatory factors MyoD and myogenin and significant downregulation of the adipogenic transcription factors peroxisome proliferator‐activated receptor‐ γ ( PPAR γ ) and CCAAT/enhancer binding protein‐ α ( CEBP α ) in masseter muscles treated with Mst‐si RNA ‐lipoplexes. The duty times of masseter muscle activity exceeding 5% showed a slight tendency to increase in both wild type and mdx mice. Therefore, cationic liposome‐mediated local administration of Mst‐si RNA could increase muscular size and improve muscle activity. Since cationic liposomes delivered si RNA to muscles effectively and are safe and cost‐effective, they may represent a therapeutic tool for use in treating muscular diseases.