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Translating human genetics into mouse: The impact of ultra‐rapid in vivo genome editing
Author(s) -
Aida Tomomi,
Imahashi Risa,
Tanaka Kohichi
Publication year - 2014
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12101
Subject(s) - genome editing , biology , gene targeting , genome , gene knockin , gene , computational biology , crispr , gene knockout , genetics , model organism , embryonic stem cell
Gene‐targeted mutant animals, such as knockout or knockin mice, have dramatically improved our understanding of the functions of genes in vivo and the genetic diversity that characterizes health and disease. However, the generation of targeted mice relies on gene targeting in embryonic stem ( ES ) cells, which is a time‐consuming, laborious, and expensive process. The recent groundbreaking development of several genome editing technologies has enabled the targeted alteration of almost any sequence in any cell or organism. These technologies have now been applied to mouse zygotes ( in vivo genome editing), thereby providing new avenues for simple, convenient, and ultra‐rapid production of knockout or knockin mice without the need for ES cells. Here, we review recent achievements in the production of gene‐targeted mice by in vivo genome editing.