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TALEN ‐induced gene knock out in D rosophila
Author(s) -
Kondo Takefumi,
Sakuma Tetsushi,
Wada Housei,
AkimotoKato Ai,
Yamamoto Takashi,
Hayashi Shigeo
Publication year - 2014
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12097
Subject(s) - transcription activator like effector nuclease , gene knockout , mutagenesis , germline , biology , gene , genetics , gene targeting , genome editing , microbiology and biotechnology , mutation , genome
We report here a case study of TALEN ‐induced gene knock out of the trachealess gene of D rosophila . Two pairs of TALEN constructs caused targeted mutation in the germ line of 39% and 17% of injected animals, respectively. In the extreme case 100% of the progeny of TALEN ‐injected fly was mutated, suggesting that highly efficient biallelic germ line mutagenesis was achieved. The mutagenic efficiency of the TALEN pairs paralleled their activity of single strand annealing ( SSA ) assay in cultured cells. All mutations were deletion of 1 to 20 base pairs. Merit and demerit of TALEN ‐based gene knockout approach compared to other genome editing technologies is discussed.