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Recent progress in genome engineering techniques in the silkworm, B ombyx mori
Author(s) -
Daimon Takaaki,
Kiuchi Takashi,
Takasu Yoko
Publication year - 2014
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12096
Subject(s) - transcription activator like effector nuclease , zinc finger nuclease , crispr , bombyx mori , genome editing , sericulture , biology , bombyx , genome engineering , genetics , mutagenesis , genome , computational biology , gene knockout , gene , mutant
Rapid advances in genome engineering tools, such as zinc finger nucleases ( ZFN s), transcription activator‐like effector nucleases ( TALEN s), and the clustered regularly interspaced palindromic repeats/ CRISPR ‐associated ( CRISPR /Cas) system, have enabled efficient gene knockout experiments in a wide variety of organisms. Here, we review the recent progress in targeted gene disruption techniques in the silkworm, B ombyx mori . Although efficiency of targeted mutagenesis was very low in an early experiment using ZFN s, recent studies have shown that TALEN s can induce highly efficient mutagenesis of desired target genes in B ombyx . Notably, mutation frequencies induced by TALEN s can reach more than 50% of G 0 gametes. Thus, TALEN s can now be used as a standard tool for gene targeting studies, even when mutant phenotypes are unknown. We also propose guidelines for experimental design and strategy for knockout experiments in B ombyx . Genome editing technologies will greatly increase the usefulness of B ombyx as a model for lepidopteran insects, the major agricultural pests, and lead to sophisticated breeding of B ombyx for use in sericulture and biotechnology.