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Antagonistic and synergistic effects of bone morphogenetic protein 2/7 and all‐trans retinoic acid on the osteogenic differentiation of rat bone marrow stromal cells
Author(s) -
Bi Wenjuan,
Gu Zhiyuan,
Zheng Yuanna,
Wang Limin,
Guo Jing,
Wu Gang
Publication year - 2013
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12090
Subject(s) - retinoic acid , bone morphogenetic protein 2 , stromal cell , chemistry , bone morphogenetic protein 7 , bone marrow , alkaline phosphatase , osteocalcin , microbiology and biotechnology , bone morphogenetic protein , regeneration (biology) , tretinoin , bone healing , biochemistry , immunology , cancer research , biology , anatomy , in vitro , gene , enzyme
The osteogenesis of bone marrow stromal cells ( BMSC s) is of paramount importance for the repair of large‐size bone defects, which may be compromised by the dietary‐accumulated all‐trans retinoic acid ( ATRA) . We have shown that heterodimeric bone morphogenetic protein 2/7 ( BMP 2/7) could induce bone regeneration in a significantly higher dose‐efficiency in comparison with homodimeric BMP s. In this study, we evaluated the effects of ATRA and BMP 2/7 on the proliferation, differentiation, mineralization and osteogenic genes. ATRA and BMP 2/7 exhibited both antagonistic and synergistic effects on the osteogenesis of BMSC s. ATRA significantly inhibited proliferation and expression of osteocalcin but enhanced the activity of alkaline phosphatase of BMSC s. On day 21, 50 ng/mL BMP 2/7 could antagonize the inhibitive effects of ATRA and significantly enhance osteogenesis of BMSC s. These findings suggested a promising application potential of heterodimeric BMP 2/7 in clinic to promote bone regeneration for the cases with dietary accumulated ATRA .